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All procedures explained below are applied in every semen analysis due to our goal to maintain our quality at the highest level.
- Sperm counting and motility are evaluated with the Makler Chamber or Neubauer Hemocytometer. Sperm concentration and motility are given by taking average after they are counted for 3 times at least.
- It is necessary to stop and colour the sperm movements in order to evaluate sperm morphology. Papanicolaou coloring technique is used to evaluate the sperm morphology in our laboratory. After 200 sperm from different sections of the preparations coloured with this method are evaluated according to the Strict Tygerberg Criteria, average is taken and morphology result is given. The most important characteristic of the Tygerberg Criteria is providence of standardization in the evaluation. The reason of calling this classification as strict criteria is the evaluation of the borderline forms as abnormal and acceptance of only flawless ones as normal.
- If morphology is evaluated by the well-educated and conscious eyes, it gives accurate results. Morphology must also be regarded as well as the sperm number and motility in patients of insemination.
- Swim-up is routinely performed in semen analysis with sufficient sperm amount. Thus, sperm concentration which will be obtained with the swim-up can be seen in the future treatments.
- It is important to distinguish the leucocyte within the semen from other circle cells. For this reason, the leucocytes can be distinguished by using Leucoscreen which is a leucocyte paint. Thus, the exact leucocyte number in the semen can be given.
- MAR (Mixed Antiglobulin Reaction) Test is a test which is applied in semen analysis in our laboratory. Due to routine application of this test which is applied on request in other laboratories because it is an expensive test, patients with immunological infertility can be detected.
In the event of existence of motile sperm in the rate of less than 75% in the semen, sperms are colored with the Vitalscreen in order to determine the rate of vital sperms. Thus, the rate of the sperms which are not motile, but vital can be exactly determined.